Pathologist & Technologies

Chemiluminescence Immunoassay (CLIA) provides a sensitive, high throughput, fully automated immunological technique, which has revolutionized Immunodiagnostics. It measures the intensity of light emitted when the complex of antigen in the patient’s sample and the enzyme linked antibody reacts with chemiluminescent molecule (such as luminol) producing a weak signal. The enzyme component then provides signal amplification.

One reason accounting for the growing popularity of chemiluminescence assays is its exquisite detection sensitivity. With its wide potential chemiluminescence has become a powerful and a versatile technology in the in vitro diagnosis. CLIA provides advantages over other methods for having increased sensitivity, less prone to interference and background signals and requires simple instrumentation.

State of the art CLIA analyzers like Siemens ADVIA Centaur, Abbott Architect i2000, Siemens Immulite 2000 and Roche EEE modular analyzers are being used for investigations.